景观规划控制构建城市特色研究

通过对欧美和日本等国景观规划控制的总结和我国景观规划控制现状的分析,促使在借鉴他国先进经验的基础上,结合我国实际情况,提出相应的手段与方法以提升我国景观规划控制水平,并展望新的目标,以有效保护和构建有特色的城市形象。     

‘鸭梨’钙调素基因的克隆与表达分析

以‘鸭梨’为材料,用RT-PCR及RACE技术克隆CaM,并借助实时定量PCR技术分析‘鸭梨’花、叶及果实不同发育时期的表达模式,获得了2个长度为764和801bp的CaM cDNA序列,分别命名为PbCaM1和PbCaM2,二者均含有450bp完整开放阅读框,编码149个氨基酸,且氨基酸序列相同。‘鸭梨’CaM在系统进化上具有高度保守性,与苹果相比,两者氨基酸序列为100%一致性。PbCaM1和PbCaM2具有时空表达特异性,它们在成叶、盛花期花瓣和幼果期表达量较高。本研究表明CaM具有高度保守性,PbCaM1和PbCaM2在‘鸭梨’果实早期生长发育过程中起着非常重要的作用。     

Establishment of Efficient Regeneration System of Populus×euramericana cl.‘Bofeng’

Compared with the species of Leuce section,the difficulty in plant regeneration during tissue culture for the species of Aigeiros section is a key limiting factor for their application in genetic engineering of forest trees.In this study,the plant growth regulators combination,copper concentration,light intensity,and the selection pressures of kanamycin were investigated using leaves of Populus×euramericana cl.’Bofeng’ as explants,and a stable and high efficient regeneration system was established.Using this system,both the shoot regeneration rate and rooting rate were up to 100%,and the average number of differentiated shoots in each leaf explant was up to 20,and the survival rate of seedlings reached 98%.The optimal selection pressure of kanamycin in shoot inducement for leaf-explant was 40 mg·L^-1,and the optimal selection pressure of kanamycin in root inducement of adventitious bud was 20 mg·L^-1.The authors also found that differentiation rate of adventitious buds of P.×euramericana cl.’Bofeng’ could be significantly promoted by increased copper concentration,and this is the first report about the key role of copper（Cu） in explant regeneration of Poplars.This regeneration system with high frequency which was established by the authors was as good as that of species of Leuce section,and it provides a good technological platform for genetic engineering of forest tree which uses the species of Aigeiros section as model materials.     

关于会计职业道德建设的探讨

个别企业中虚假的会计信息已严重干扰了市场经济发展,扰乱了市场秩序,败坏了社会风气,这就使会计职业道德问题显现出来。本文浅述了会计职业道德内容、现状及其重要性,从而提出了关于加强会计职业道德建设的几点建议。     

从教育券到教育卡：农民工随迁子女入学问题破解新思路

农民工随迁子女教育问题是当前一个亟待解决的社会难题。由于国家实施＂两为主＂政策,致使流入地政府承受着巨大的财政压力,在解决农民工随迁子女教育问题上心有余而力不足。而近年来引进的＂教育券＂制度,在实际工作中也存在一定的局限性。基于由国家、流出地政府、流入地政府按一定比例共同承担农民工随迁子女义务教育经费的理论,提出的＂教育卡＂新构想,则可以从操作层面较好地解决教育经费承担的一系列问题。     

从个案调查辨析改进院校图书馆管理服务

通过对某专业院校图书馆文献借阅情况调查,就改进院校图书馆管理,优化读者服务谈了一些看法。侧重于研究教学型的专业院校图书馆作讨论。     

昆明市东川区资源枯竭的困境和经济转型的思考

资源的开采和利用在给人们带来财富和享受的同时,也因为资源的不断耗尽与环境的破坏而给人们带来了大量的灾难和问题。云南东川作为新中国第一批因矿而建的城市,在经过多年对矿产资源的开采和利用后,随着资源的枯竭而使其经济陷入极端困境之中。通过对东川经济转型期如何改变资源依赖路径,形成多元产业协调发展,以及如何实现生态环境保护与经济发展相协调的长效机制等问题进行了探讨,并提出相应的对策建议。     

构建高校图书馆与农家书屋的协作机制推动区域经济持续发展

从活跃农民群众文化生活、发展和繁荣农村文化的角度出发，从促进社会主义农村经济的协调发展、全面提高农民综合素质入手，对当前“农家书屋”发展现状以及存在的问题进行分析，探讨了高校图书馆对“农家书屋”工程建设的优势和促进“农家书屋”工程建设的有效方法和途径。     

Floristic diversity and vegetation patterns along disturbance gradient in arid coasts in southern Mediterranean: Case of the Gulf of Gabès,southern Tunisia

Knowledge about vegetation patterns along disturbance gradients is essential for conservation and management of coastal habitats in arid regions. This study focuses on the floristic diversity and the factors controlling the vegetation structure in the Gulf of Gabès (Tunisia). For this purpose, the floristic diversity of 120 relevés belonging to three coastal sites with different disturbance levels (40 relevés per site) was analyzed in relation with 10 environmental and anthropogenic factors. Therophytes constitute 54% of the 237 plants species belonging to 44 families identified in the present study. The species richness and floristic diversity indices exhibited a similar trend and peaked in the slightly disturbed site (H′?=?3.38; S?=?176). The similarity between sites was relatively low and reached its maximum between the little and the highly disturbed sites. Two-way indicator species analysis and detrended correspondence analysis applied on the floristic data resulted in groups mainly determined by disturbance level by explicitly segregating the three sites. Canonical correspondence analysis (CCA) indicated that the spatial pattern of species composition was mainly influenced by anthropogenic variables. Partial CCA revealed that the effect of anthropogenic variables on floristic composition was thrice higher than the effect of environmental variables (57.5% vs. 18.4%, respectively). Overall, this study provides a broad understanding of the floristic diversity and vascular plant richness in the Gulf of Gabès. The obtained results reflected a disturbance–diversity pattern and thus revealed the obvious importance of maintaining the intermediate disturbance for biodiversity conservation and monitoring.     

Phytogeny and Cross-infectivity of Powdery Mildew Isolates (Podosphaera fuliginea s. lat.) on Cosmos and Cucumber

To clarify the relationship between the phylogeny and infectivity of isolates of Podosphaera fuliginea s. lat. (= Sphaerotheca fuliginea s. lat.) from cosmos and cucumber, more than 50 powdery mildew isolates from these two plants were subjected to nucleotide sequencing or PCR-RFLP analysis of the rDNA internal transcribed spacer (ITS) region and cross-inoculation tests. The isolates from both cosmos and cucumber are genetically monotypic, and there are six nucleotide substitutions in the rDNA ITS region between isolates from cosmos and cucumber. Cross-inoculation tests of these isolates revealed that isolates from cosmos are not pathogenic on cucumber. Although isolates from cucumber produced conidia on leaves of cosmos in the laboratory, the conidial density was much lower than that from isolates from cosmos. This result, as well as the fact that the cucumber strain was not isolated from cosmos in fields, suggests that isolates from cucumber do not infect cosmos in the field. Therefore, powdery mildews on cosmos and cucumber can be regarded to have become specialized for their hosts both genetically and pathogenically. The present study reconfirms the close relationship between phylogeny and infectivity of powdery mildew fungi. Host specialization may be a trigger that causes genetic divergence of powdery mildew fungi. Received 28 June 2000/ Accepted in revised form 4 September 2000